ABSTRACT
Chronic exposure to tobacco dust causes nasal inhalation and cutaneous absorption of tobacco alkaloids especially nicotine, therefore the considerable evidences showed that workers employed in bidi industries are at risk of cancer, lung diseases and other many health related common problems. Many references revealed that tobacco dust exposure induces mutations, damage to DNA etc. which are supposed to be the consequences of free radical generation. In present study attempt have been made to evaluate the status of oxidants and antioxidants and their relation with nicotine. 90 bidi workers were screened for serum lipid peroxide (MDA) and serum nitric oxide (NO*) as oxidant and erythrocytic-Superoxide Dismutase (RBC-SOD), Vitamin-C as antioxidant. Total antioxidant capacity (TAC) and urinary cotinine were also measured. These bidi workers were further divided in 3 groups, Group 1, 11 and III exposed to tobacco dust for about 10 to 14 yrs, 15 to 19 yrs and 20 to 24 yrs of exposure respectively. Highly significant excretion of Urinary cotinine was found in all groups of bidi workers as compared with control (p < 0.001). The levels of MDA, and NO* were found to be significantly elevated in all the three groups with progression of exposure, than the control (p < 0.001), where as the levels of RBC-SOD, Vitamin-C and TAC were significantly decreased in all the three groups as compared with controls (p < 0.001). From our findings it is evident that nicotine absorption might contribute to the disturbed oxidant and antioxidant balance leading to oxidative stress.